High performance liquid chromatograph is widely used in fields including food, medicine, chemical industry, life, environment, etc. As a general analysis and testing equipment, it is widely applied in universities, scientific research institutes, hospitals, regulatory agencies, factories and enterprises. In order to improve the working efficiency of the liquid chromatograph and prolong its service life, how to use the chromatograph correctly?

Correct operation of lab high performance liquid chromatograph:

1. Check whether the solvent on the solvent tray is sufficient. It is advisable that the liquid level of the solvent exceeds the filter head of the infusion tube by more than 5 cm.

2. Check whether there are air bubbles inside the infusion tube. If there is, it should be discharged through the drain valve in time.

3. Solvents and samples should be processed, filtered, and degassed.

4. Turn on the power of the host, then turn on the power of the detector, pump A, pump B, and oven in turn.

5. Turn on the computer and start the chromatography workstation.

6. First turn on the piston pump in the workstation and equilibrate the system with the desired mobile phase (about 30 minutes).

7. Turn on the deuterium lamp and wait for the system baseline to stabilize.

8. Start the test.

High performance liquid chromatography is convenient and quick to use in qualitative and quantitative analysis such as substance identification and content detection, and has wide applicability to samples, and is not limited by the volatility and thermal stability of the analyzed object. Therefore, high performance liquid chromatograph has a very wide range of applications.