The calibration of any instrument is to make it work more accurately. The UV spectrophotometer is an analytical instrument based on the principle of UV-visible spectrophotometry, which uses the radiation absorption of material molecules in the UV-visible spectral region to analyze. Its calibration must meet certain specifications.

How to calibrate laboratory UV spectrophotometer:

1. Before calibration, UV spectrophotometer should be turned on and warmed up for enough time to reach stabilization, generally at least 30 minutes.

2. When selecting standard substances, it is necessary to note that the calibration should be performed under the conditions of recurring normative values (i.e. the operating conditions of the normative substance rules), otherwise wrong calibration results will be produced.

3. When using standard solutions to calibrate, special attention must also be paid to the laboratory temperature during calibration and measurement, because these standard substances have different transmittance standard values at different temperatures.

4. The standard substance used for the standard must be stored under the conditions stipulated in the manual. For example, the interference filter should be kept in a dry and clean environment to prevent the surface from being polluted, damp, unglued or mildewed. All standard substances should be used within the validity period indicated on the certificate.

5. The uncertainty of the standard value of the neutral filter selected for the ultraviolet spectrophotometer must meet the transfer standard. When calibrating, pay attention to the directivity of the standard filter.