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Calibration Method of UV Spectrophotometer

Nov 14,2024

Ultraviolet spectrophotometer is a commonly used photometer product that can arbitrarily select light of different wavelengths in the ultraviolet and visible light region. It has the advantages of stable performance, flexible use, high reliability and easy maintenance.

 visible spectrophotometer

The UV spectrophotometer needs to be calibrated when in use. The following is the calibration method:

One of the most important physical and chemical quantities in spectrophotometry is absorbance. In order to obtain accurate research results, it is very important to accurately measure the absorbance of the sample solution. Generally, the unreliability of the analysis results is related to accidental errors and systematic errors. Accidental errors affect the precision of the measurement and can be reduced by statistical processing of a sufficient number of measurements. Systematic errors affect the accuracy of the measurement results. Under roughly the same experimental conditions, the accurate measurement results of a substance can be compared to unify the systematic errors.

 micro spectrophotometer

The influence of the systematic error and operation error of the spectrophotometer on the measured absorbance can be checked and corrected. Regarding the operation error, in most cases, the occurrence of such error can be controlled or reduced by strictly following the operating procedures, zeroing the instrument, and accurately weighing. About the systematic error, it can be overcome by regular calibration of the spectrophotometer. If a very high accuracy measurement is required, it must be calibrated every day.