The uncertainty of the spectrophotometer wavelength accuracy test can be reduced from the following aspects:

I. Instrument

1. Choose high-quality instruments

Purchase a spectrophotometer with high accuracy and good stability. Check the technical parameters of the instrument and select equipment that meets the test requirements and has excellent performance.

2. Regular maintenance

Perform regular maintenance and calibration according to the instrument manual. Regularly calibrate the wavelength of the instrument to ensure the stability of the wavelength accuracy during long-term use.

3. Control environmental conditions

Place the spectrophotometer in a stable environment and control the temperature and humidity within a suitable range.

II. Standard materials

1. Select appropriate standard materials

Select standard materials with accurate known wavelength values and good stability. Select appropriate standard materials according to the working wavelength range of the lab spectrophotometer to ensure that the wavelength of the standard materials covers the test range.

2. Correct use and storage of standard materials

Operate according to the instructions for use of the standard materials to avoid affecting the accuracy of their wavelengths due to improper use. Properly store standard materials to avoid being affected by environmental factors such as temperature, humidity, and light. Standard materials can be stored in a dry, light-proof, and constant temperature environment to extend their service life and maintain wavelength stability.

III. Operation

1. Standardize the operating process

Operators should strictly follow the operating manual of the spectrophotometer to avoid frequent adjustments to instrument parameters or moving samples during operation to reduce uncertainty caused by human factors.

2. Improve operator skills

Provide professional training for operators to familiarize them with the principles, operating methods, and maintenance knowledge of the spectrophotometer.

IV. Data processing

1. Take the average value of multiple measurements:

Measure the same standard substance or sample repeatedly, and then take the average value as the measurement result. This can reduce the impact of random errors and improve the accuracy and stability of the measurement.

2. Reasonable data processing method:

Choose a suitable data processing method to avoid introducing additional uncertainty due to improper data processing.